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phi x control  (Illumina Inc)


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    Structured Review

    Illumina Inc phi x control
    Phi X Control, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 3823 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phi+x+control/PhiX+Control+v3/bio_rxiv__64898__2026__03__13__711679-87-18-20
    Average 99 stars, based on 3823 article reviews
    phi x control - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Control:

    Article Title: Structural and Conformational Impact of Deleterious Spike Protein Mutations in SARS-CoV-2 Omicron Lineages
    Article Snippet: Sample library normalization and MiSeq sample loading were carried out according to the Illumina Library Prep protocol. .. All normalized libraries were pooled and spiked with Phi-X control at a final concentration of 0.1 pM followed by loading onto a Mi-Seq reagent kit V3 600 cycle (Illumina, Portland, USA) [ ] and sequenced on a MiSeq Platform (Illumina, Portland, USA). .. Raw reads from Illumina sequencing were assembled using Viralrecon [ ]and results were further validated using in-house pipeline.

    Article Title: Use of Whole-Genome Sequencing in the Investigation of a Nosocomial Influenza Virus Outbreak.
    Article Snippet: Sample library normalization and MiSeq sample loading were carried out according to the Nextera XT protocols. .. Pooled normalized samples, including a Phi-X control at a final concentration of 0.1 pM, were loaded onto a Mi-Seq reagent kit V3 600 cycle (Illumina) and sequenced on a MiSeq (Illumina). .. Consensus genomes were generated from short reads, using ICONIC’s in-house de novo assembly pipeline, applying a read depth cutoff of ≥20 reads to the final sequences.

    Article Title: Combinatorial Strategies for Improving Multiple-Stress Resistance in Industrially Relevant Escherichia coli Strains
    Article Snippet: Buffer HT1 (Illumina, San Diego, CA) was added up to a final volume of 570 μM. .. Due to the Tn 5 libraries being low-diversity libraries due to many bases of anticipated identical sequence at the 5′ terminus, 30 μl of Phi-X control (Illumina) was added to the pooled libraries, and the entire volume was loaded onto the flow cell provided in the MiSeq Reagent kit v2, subjected to 300 cycles (Illumina), and sequenced on a MiSeq sequencing system. ..

    Article Title: Energy Flux Regulates Cell Death Induced by California Serogroup Orthobunyaviruses
    Article Snippet: Sequencing was performed by Dartmouth Genomics Shared Resource Core facilities on an Illumina MiniSeq using high output 75 or 150 cycle run with single end reads. .. Read count was a minimum of 50 reads per sgRNA or 3.8 million reads per library copy; 10% Phi-X Control (Illumina, catalog #FC-110-3001) was added to increase diversity. ..

    Concentration Assay:

    Article Title: Structural and Conformational Impact of Deleterious Spike Protein Mutations in SARS-CoV-2 Omicron Lineages
    Article Snippet: Sample library normalization and MiSeq sample loading were carried out according to the Illumina Library Prep protocol. .. All normalized libraries were pooled and spiked with Phi-X control at a final concentration of 0.1 pM followed by loading onto a Mi-Seq reagent kit V3 600 cycle (Illumina, Portland, USA) [ ] and sequenced on a MiSeq Platform (Illumina, Portland, USA). .. Raw reads from Illumina sequencing were assembled using Viralrecon [ ]and results were further validated using in-house pipeline.

    Article Title: Use of Whole-Genome Sequencing in the Investigation of a Nosocomial Influenza Virus Outbreak.
    Article Snippet: Sample library normalization and MiSeq sample loading were carried out according to the Nextera XT protocols. .. Pooled normalized samples, including a Phi-X control at a final concentration of 0.1 pM, were loaded onto a Mi-Seq reagent kit V3 600 cycle (Illumina) and sequenced on a MiSeq (Illumina). .. Consensus genomes were generated from short reads, using ICONIC’s in-house de novo assembly pipeline, applying a read depth cutoff of ≥20 reads to the final sequences.

    Sequencing:

    Article Title: Combinatorial Strategies for Improving Multiple-Stress Resistance in Industrially Relevant Escherichia coli Strains
    Article Snippet: Buffer HT1 (Illumina, San Diego, CA) was added up to a final volume of 570 μM. .. Due to the Tn 5 libraries being low-diversity libraries due to many bases of anticipated identical sequence at the 5′ terminus, 30 μl of Phi-X control (Illumina) was added to the pooled libraries, and the entire volume was loaded onto the flow cell provided in the MiSeq Reagent kit v2, subjected to 300 cycles (Illumina), and sequenced on a MiSeq sequencing system. ..



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol for microbial profiling of low-biomass upper respiratory tract samples

    doi: 10.1016/j.xpro.2025.103740

    Figure Lengend Snippet:

    Article Snippet: Check the Phi-X percentage using Illumina MiSeq Viewer.

    Techniques: Virus, Recombinant, Control, Real-time Polymerase Chain Reaction, Binding Assay, Lysis, Magnetic Beads, Picogreen Assay, Sequencing, Software

    Journal: STAR Protocols

    Article Title: Protocol for microbial profiling of low-biomass upper respiratory tract samples

    doi: 10.1016/j.xpro.2025.103740

    Figure Lengend Snippet:

    Article Snippet: Phi-X control v.3 , Illumina, CA, USA , Cat#FC-110-3001.

    Techniques: Virus, Recombinant, Control, Real-time Polymerase Chain Reaction, Binding Assay, Lysis, Magnetic Beads, Picogreen Assay, Sequencing, Software